Composite

Part:BBa_K4307006:Design

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)


attB-promoter-attP & Ser integrase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 667
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 361
    Illegal NheI site found at 350
    Illegal PstI site found at 667
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 361
    Illegal BglII site found at 525
    Illegal BamHI site found at 289
    Illegal BamHI site found at 549
    Illegal BamHI site found at 927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 667
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 667
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 1266
    Illegal AgeI site found at 124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24
    Illegal BsaI.rc site found at 1933
    Illegal SapI site found at 106


Design Notes

We successfully expressed the integrase from bacteriophage in bacteria, and the results of the fluorescence assay showed that integrase could have obvious inversion effect, but the action time was a little bit long.


Source

E.coli

References